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Experiment Overview

Repository ID: FR-FCM-ZZAG Experiment name: Effects of CA on Mouse Treg Differentiation MIFlowCyt score: 81.11%
Primary researcher: Margaret Lowe PI/manager: Margaret Lowe Uploaded by: Margaret Lowe
Experiment dates: 2013-02-08 - 2013-03-06 Dataset uploaded: Mar 2014 Last updated: Aug 2014
Keywords: [FoxP3] [mouse T cells] [CA] [Treg] [TGFb] Manuscripts: [24498387] [PMC3912126] Plosonelogo
Organizations: University of California, San Francisco, San Francisco, CA (United States)
Purpose: To determine the effects of CA on FOXP3 expression in mouse naive T cells stimulated with or without TGFbeta
Conclusion: CA does not increase mouse Treg differentiation.
Comments: See figure 6D of Lowe et al Plos One 2014
Funding: Not disclosed
Quality control: None


Experiment variables

Doses
· 10 µM Treg_CA10 b0.fcs · Treg_CA10 b0.fcs-(1) · Treg_CA10 b10.fcs · Treg_CA10 b10.fcs-(1) · Treg_CA10 b5.fcs · Treg_CA10 b5.fcs-(1) · beta 0_CA 10.fcs · beta 0_CA 10.fcs-(1) · beta 10_CA 10.fcs · beta 10_CA 10.fcs-(1) · beta 5_CA 10.fcs · beta 5_CA 10.fcs-(1)
· 25 µM Treg_CA 25 b0.fcs · Treg_CA 25 b0.fcs-(1) · Treg_CA 25 b10.fcs · Treg_CA 25 b10.fcs-(1) · Treg_CA 25 b5.fcs · Treg_CA 25 b5.fcs-(1) · beta 0_CA 25.fcs · beta 0_CA 25.fcs-(1) · beta 10_CA 25.fcs · beta 10_CA 25.fcs-(1) · beta 5_CA 25.fcs · beta 5_CA 25.fcs-(1)
· vehicle Treg_D B0.fcs · Treg_D B0.fcs-(1) · Treg_D B10.fcs · Treg_D B10.fcs-(1) · Treg_D B5.fcs · Treg_D B5.fcs-(1) · beta 0_DMSO.fcs · beta 0_DMSO.fcs-(1) · beta 10_DMSO.fcs · beta 10_DMSO.fcs-(1) · beta 5_DMSO.fcs · beta 5_DMSO.fcs-(1)

Sample Type
· CA Treg_CA 25 b0.fcs · Treg_CA 25 b0.fcs-(1) · Treg_CA 25 b10.fcs · Treg_CA 25 b10.fcs-(1) · Treg_CA 25 b5.fcs · Treg_CA 25 b5.fcs-(1) · Treg_CA10 b0.fcs · Treg_CA10 b0.fcs-(1) · Treg_CA10 b10.fcs · Treg_CA10 b10.fcs-(1) · Treg_CA10 b5.fcs · Treg_CA10 b5.fcs-(1) · beta 0_CA 10.fcs · beta 0_CA 10.fcs-(1) · beta 0_CA 25.fcs · beta 0_CA 25.fcs-(1) · beta 10_CA 10.fcs · beta 10_CA 10.fcs-(1) · beta 10_CA 25.fcs · beta 10_CA 25.fcs-(1) · beta 5_CA 10.fcs · beta 5_CA 10.fcs-(1) · beta 5_CA 25.fcs · beta 5_CA 25.fcs-(1)
· DMSO Treg_D B0.fcs · Treg_D B0.fcs-(1) · Treg_D B10.fcs · Treg_D B10.fcs-(1) · Treg_D B5.fcs · Treg_D B5.fcs-(1) · beta 0_DMSO.fcs · beta 0_DMSO.fcs-(1) · beta 10_DMSO.fcs · beta 10_DMSO.fcs-(1) · beta 5_DMSO.fcs · beta 5_DMSO.fcs-(1)

Conditions
· No TGFβ Treg_CA 25 b0.fcs · Treg_CA 25 b0.fcs-(1) · Treg_CA10 b0.fcs · Treg_CA10 b0.fcs-(1) · Treg_D B0.fcs · Treg_D B0.fcs-(1) · beta 0_CA 10.fcs · beta 0_CA 10.fcs-(1) · beta 0_CA 25.fcs · beta 0_CA 25.fcs-(1) · beta 0_DMSO.fcs · beta 0_DMSO.fcs-(1)
· 5 ng/mL TGFβ Treg_CA 25 b5.fcs · Treg_CA 25 b5.fcs-(1) · Treg_CA10 b5.fcs · Treg_CA10 b5.fcs-(1) · Treg_D B5.fcs · Treg_D B5.fcs-(1) · beta 5_CA 10.fcs · beta 5_CA 10.fcs-(1) · beta 5_CA 25.fcs · beta 5_CA 25.fcs-(1) · beta 5_DMSO.fcs · beta 5_DMSO.fcs-(1)
· 10 ng/mL TGFβ Treg_CA 25 b10.fcs · Treg_CA 25 b10.fcs-(1) · Treg_CA10 b10.fcs · Treg_CA10 b10.fcs-(1) · Treg_D B10.fcs · Treg_D B10.fcs-(1) · beta 10_CA 10.fcs · beta 10_CA 10.fcs-(1) · beta 10_CA 25.fcs · beta 10_CA 25.fcs-(1) · beta 10_DMSO.fcs · beta 10_DMSO.fcs-(1)

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