Experiment Overview
| Repository ID: | FR-FCM-Z25K | Experiment name: | Dual upregulation of CD71 and CD98 versus CFSE | MIFlowCyt score: | 70.00% |
| Primary researcher: | George Elias | PI/manager: | George Elias | Uploaded by: | George Elias |
| Experiment dates: | 2017-07-15 - 2017-08-15 | Dataset uploaded: | Jul 2019 | Last updated: | Jul 2019 |
| Keywords: | [VZV lyzate] [hepatitis B surface antigen] [isopentenyl pyrophosphate] [CD71] [CD98] | Manuscripts: | [31356002] |
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| Organizations: |
Antwerp University, Laboratory of Experimental Hematology, Antwerp, Antwerp (Belgium)
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| Purpose: | To investigate the overlap between dual upregulation of CD71 and CD98 on T cells and the loss of CFSE fluorescence | ||||
| Conclusion: | Dual upregulation of CD71 and CD98 T cells identifies actively proliferating T cells after antigenic stimulus, as identified by the loss of CFSE fluorescence | ||||
| Comments: | Events in the files uploaded have been gating on for viable CD3+ singlets to decrease the size of files. | ||||
| Funding: | University of Antwerp [BOF Concerted Research Action (PS ID 30730), Methusalem funding] and the Research Foundation Flanders | ||||
| Quality control: | BD CS&T beads were used to provide a standardized method to perform quality control of the instrument’s optics and fluidics. Compensation controls were used for automatic compensation on FlowJo Negative control were used to set gates for CD71, CD98 and CFSE (cells left unstimulated) | ||||
Experiment variables
| Conditions | |
|---|---|
| · NC | D01_aNC_DD.fcs · D02_aNC_DD.fcs · D03_aNC_DD.fcs · D04_aNC_DD.fcs |
| · PC | D01_PC_DD.fcs · D02_PC_DD.fcs · D03_PC_DD.fcs · D04_PC_DD.fcs |
| · IPP_IL2 | D01_IPP + IL2_DD.fcs · D02_IPP + IL2_DD.fcs · D03_IPP + IL2_DD.fcs · D04_IPP + IL2_DD.fcs |
| · VZV_Lysate | D01_VZV Lysate_DD.fcs · D02_VZV Lysate_DD.fcs · D03_VZV Lysate_DD.fcs · D04_VZV Lysate_DD.fcs |
| · CMV_pp65 | D01_CMV pp65_DD.fcs · D02_CMV pp65_DD.fcs · D03_CMV pp65_DD.fcs · D04_CMV pp65_DD.fcs |
| · HBVac | D01_HBVac_DD.fcs · D02_HBVac_DD.fcs · D03_HBVac_DD.fcs · D04_HBVac_DD.fcs |
| Individuals | |
|---|---|
| · D01 | D01_CMV pp65_DD.fcs · D01_HBVac_DD.fcs · D01_IPP + IL2_DD.fcs · D01_PC_DD.fcs · D01_VZV Lysate_DD.fcs · D01_aNC_DD.fcs |
| · D02 | D02_CMV pp65_DD.fcs · D02_HBVac_DD.fcs · D02_IPP + IL2_DD.fcs · D02_PC_DD.fcs · D02_VZV Lysate_DD.fcs · D02_aNC_DD.fcs |
| · D03 | D03_CMV pp65_DD.fcs · D03_HBVac_DD.fcs · D03_IPP + IL2_DD.fcs · D03_PC_DD.fcs · D03_VZV Lysate_DD.fcs · D03_aNC_DD.fcs |
| · D04 | D04_CMV pp65_DD.fcs · D04_HBVac_DD.fcs · D04_IPP + IL2_DD.fcs · D04_PC_DD.fcs · D04_VZV Lysate_DD.fcs · D04_aNC_DD.fcs |
