Experiment Overview
| Repository ID: | FR-FCM-Z28F | Experiment name: | Immunophenotyping leukocytes in small volume pediatric samples | MIFlowCyt score: | 45.50% |
| Primary researcher: | Dawid Swieboda | PI/manager: | Dawid Swieboda | Uploaded by: | Dawid Swieboda |
| Experiment dates: | 2017-10-01 - 2018-05-31 | Dataset uploaded: | Aug 2019 | Last updated: | Nov 2019 |
| Keywords: | [] [OMIP] [CyTOF; mass cytometry; innate immunity] [respiratory] [Innate Lymphoid Cells (ILCs)] | Manuscripts: | [31633878] |
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| Organizations: |
ICL, NHLI, London, (UK)
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| Purpose: | Establish protocols for ILC enumeration in respiratory and blood samples and to demonstrate the feasibility of detecting ILCs in small volumes of paediatric samples. | ||||
| Conclusion: | In conclusion, the study has optimised protocols for enumerating ILCs (NK cells, ILC1, ILC2, ILC3) and granulocytes (eosinophils and neutrophils). The detection of ILCs in small volumes of whole blood and respiratory paediatric samples was successfully demonstrated. Finally, the impact of different protocols of cryopreservation and stabilisation of whole blood and airways cells was assessed. Our data clearly show that innate lymphocytes from paediatric samples must be processed fresh, so this will be applied to all samples collected in the future. These enabling studies allow us to now progress to studies directly investigating the importance of ILCs in controlling and maintaining immune homeostasis during bronchiolitis. | ||||
| Comments: | OMIP | ||||
| Funding: | Asthma UK Centre in Allergic Mechanisms of Asthma, Imperial College London; Grant number: AUK-BC-2015-01; Imperial College Healthcare NHS Trust- Biomedical Research Centre (BRC) Funding; Grant number: RDA02. | ||||
| Quality control: | CST beads ABC total compensation beads FMOs Antody titrations | ||||
