Experiment Overview
| Repository ID: | FR-FCM-Z2KE | Experiment name: | MDMs LPS MP | MIFlowCyt score: | 93.53% |
| Primary researcher: | Barbara Drasler | PI/manager: | Barbara Drasler | Uploaded by: | Barbara Drasler |
| Experiment dates: | 2019-12-19 - 2020-02-05 | Dataset uploaded: | Apr 2020 | Last updated: | May 2020 |
| Keywords: | [LPS] [macrophages] [methylprednisolone] | Manuscripts: | |||
| Organizations: |
Adolphe Merkle Institute, University of Fribourg, Fribourg, Fribourg (Switzerland)
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| Purpose: | To assess MDMs shifts after treatments with LPS and MP for the anti-inflammatory model paper to be submitted to the special issue of the journal Frontiers. | ||||
| Conclusion: | As differentiated MDMs have very high CD206 expression. Incubation with LPS for 48h reduces CD206 expression and only slightly increases CD86 expression. Methylprednisolone only slightly shifts reduces CD206 expression in 24h when in co-presence with LPS | ||||
| Comments: | LPS was applied first for 24 h and then methylprednisolone was added for 24h, i.e. LPS was not removed before the addition of the drug. The growth factor, i.e. M-CSF, was removed before the addition of LPS | ||||
| Funding: | Adolphe Merkle Fundation and Peter and Traudl Engelhorn | ||||
| Quality control: | Unstained samples for every sample | ||||
