Experiment Overview
| Repository ID: | FR-FCM-Z6G4 | Experiment name: | T cell panel development patient vs control | MIFlowCyt score: | 63.22% |
| Primary researcher: | Jack Edwards | PI/manager: | Jack Edwards | Uploaded by: | Jack Edwards |
| Experiment dates: | 2021-11-04 - 2022-04-07 | Dataset uploaded: | Jun 2023 | Last updated: | Jun 2023 |
| Keywords: | [absolute cell counting] [IMMUNOPHENOTYPE] [melanoma] [spectral flow cytometry] [T-cell] | Manuscripts: | [37692904] [PMC10484688] | ||
| Organizations: |
Monash University, Department of Immunology, Melbourne, Victoria (Australia)
|
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| Purpose: | Development, optimisation, and application of two spectral flow cytometry panels to a cohort of melanoma patients and healthy controls. Also includes a batch control reference sample across 11 batches. Panels assessed (1) resting T cell phenotype and (2) CD3/CD28 stimulated T cell phenotype. | ||||
| Conclusion: | Panels were successfully developed. Application to patient and control cohorts demonstrated significant differences in T cell phenotype between groups. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Daily Cytek Aurora QC as per manufacturer recommendations. | ||||
Experiment variables
| Sample Type | |
|---|---|
| · Patient | ACB001-01_BCDM1044_MCT1.fcs · ACB001-01_BCDM1044_MCT2_stim.fcs · ACB003-01_BCDM1057_MCT1.fcs · ACB003-01_BCDM1057_MCT2_stim.fcs · ACB005-01_BCDM1062_MCT1.fcs · ACB005-01_BCDM1062_MCT2_stim.fcs · ACB007-01_BCDM1067_MCT1.fcs · ACB007-01_BCDM1067_MCT2_stim.fcs · ACB011-01_BCDM1093_MCT1.fcs · ACB011-01_BCDM1093_MCT2_stim.fcs · ACB013-01_BCDM1104_MCT1.fcs · ACB013-01_BCDM11104_MCT2_stim.fcs · ACB014-01_BCDM1110_MCT1.fcs · ACB014-01_BCDM1110_MCT2_stim.fcs · ACB015-01_BCDM1112_MCT2_stim.fcs · ACB017-01_BCDM1116_MCT1.fcs · ACB017-01_BCDM1116_MCT2_stim.fcs · ACB018-01_BCDM1119_MCT1.fcs · ACB018-01_BCDM1119_MCT2_stim.fcs · ACB019-01_BCDM1123_MCT1.fcs · ACB019-01_BCDM1123_MCT2_stim.fcs · ACB021-01_BCDM1139_MCT1.fcs · ACB021-01_BCDM1139_MCT2_stim.fcs · ACB024-01_BCDM1194_MCT1.fcs · ACB024-01_BCDM1194_MCT2_stim.fcs · ACB038-01_BCDM1535_MCT1.fcs · ACB038-01_BCDM1535_MCT2_stim.fcs · ACB039-01_BCDM1585_MCT1.fcs · ACB039-01_BCDM1585_MCT2_stim.fcs · ACB040-01_BCDM1595_MCT1.fcs · ACB040-01_BCDM1595_MCT2_stim.fcs · ACB015-01_BCDM1112_MCT1.fcs |
| · Healthy control | HCM109_BCDM1433_MCT1.fcs · HCM109_BCDM1433_MCT2_stim.fcs · HCM114_BCDM1432_MCT1.fcs · HCM114_BCDM1432_MCT2_stim.fcs · HCM116_BCDM1447_MCT1.fcs · HCM116_BCDM1447_MCT2_stim.fcs · HCM117_BCDM1451_MCT1.fcs · HCM117_BCDM1451_MCT2_stim.fcs · HCM118_BCDM1452_MCT1.fcs · HCM118_BCDM1452_MCT2_stim.fcs · HCM119_BCDM1476_MCT1.fcs · HCM119_BCDM1476_MCT2_stim.fcs · HCM120_BCDM1481_MCT1.fcs · HCM120_BCDM1481_MCT2_stim.fcs · HCM121_BCDM1487_MCT1.fcs · HCM121_BCDM1487_MCT2_stim.fcs · HCM122_BCDM1492_MCT1.fcs · HCM122_BCDM1492_MCT2_stim.fcs · HCM123_BCDM1537_MCT1.fcs · HCM123_BCDM1537_MCT2_stim.fcs · HCM125_BCDM1680_MCT1.fcs · HCM125_BCDM1680_MCT2_stim.fcs · HCM126_BCDM1754_MCT1.fcs · HCM126_BCDM1754_MCT2_stim.fcs · HCM127_BCDM1755_MCT1.fcs · HCM127_BCDM1755_MCT2_stim.fcs · HCM128_BCDM1756_MCT1.fcs · HCM128_BCDM1756_MCT2_stim.fcs · HCM129_BCDM1804_MCT1.fcs · HCM129_BCDM1804_MCT2_stim.fcs · HCM130_BCDM1873_MCT1.fcs · HCM130_BCDM1873_MCT2_stim.fcs |
| · Batch control | BBC017_20211109_MCT1.fcs · BBC017_20211109_MCT2_stim.fcs · BBC017_20211117_MCT1.fcs · BBC017_20211117_MCT2_stim.fcs · BBC017_20211207_MCT1.fcs · BBC017_20211207_MCT2_stim.fcs · BBC017_20211215_MCT1.fcs · BBC017_20211215_MCT2_stim.fcs · BBC017_20211217_MCT1.fcs · BBC017_20211217_MCT2_stim.fcs · BBC017_20211222_MCT1.fcs · BBC017_20211222_MCT1_2.fcs · BBC017_20211222_MCT2_stim_2.fcs · BBC017_20211222_MCT2_stim_2.fcs-(1) · BBC017_20220302_MCT1.fcs · BBC017_20220302_MCT2_stim.fcs · BBC017_20220304_MCT1.fcs · BBC017_20220304_MCT2_stim.fcs · BBC017_20220318_MCT1.fcs · BBC017_20220318_MCT2_stim.fcs · BBC017_MCT2_stim_20211104.fcs · MCT1_BBC017_20211104.fcs |
| Conditions | |
|---|---|
| · Resting | ACB001-01_BCDM1044_MCT1.fcs · ACB003-01_BCDM1057_MCT1.fcs · ACB005-01_BCDM1062_MCT1.fcs · ACB007-01_BCDM1067_MCT1.fcs · ACB011-01_BCDM1093_MCT1.fcs · ACB013-01_BCDM1104_MCT1.fcs · ACB014-01_BCDM1110_MCT1.fcs · ACB017-01_BCDM1116_MCT1.fcs · ACB018-01_BCDM1119_MCT1.fcs · ACB019-01_BCDM1123_MCT1.fcs · ACB021-01_BCDM1139_MCT1.fcs · ACB024-01_BCDM1194_MCT1.fcs · ACB038-01_BCDM1535_MCT1.fcs · ACB039-01_BCDM1585_MCT1.fcs · ACB040-01_BCDM1595_MCT1.fcs · BBC017_20211109_MCT1.fcs · BBC017_20211117_MCT1.fcs · BBC017_20211207_MCT1.fcs · BBC017_20211215_MCT1.fcs · BBC017_20211217_MCT1.fcs · BBC017_20211222_MCT1.fcs · BBC017_20211222_MCT1_2.fcs · BBC017_20220302_MCT1.fcs · BBC017_20220304_MCT1.fcs · BBC017_20220318_MCT1.fcs · HCM109_BCDM1433_MCT1.fcs · HCM114_BCDM1432_MCT1.fcs · HCM116_BCDM1447_MCT1.fcs · HCM117_BCDM1451_MCT1.fcs · HCM118_BCDM1452_MCT1.fcs · HCM119_BCDM1476_MCT1.fcs · HCM120_BCDM1481_MCT1.fcs · HCM121_BCDM1487_MCT1.fcs · HCM122_BCDM1492_MCT1.fcs · HCM123_BCDM1537_MCT1.fcs · HCM125_BCDM1680_MCT1.fcs · HCM126_BCDM1754_MCT1.fcs · HCM127_BCDM1755_MCT1.fcs · HCM128_BCDM1756_MCT1.fcs · HCM129_BCDM1804_MCT1.fcs · HCM130_BCDM1873_MCT1.fcs · MCT1_BBC017_20211104.fcs · ACB015-01_BCDM1112_MCT1.fcs |
| · Stimulated | ACB001-01_BCDM1044_MCT2_stim.fcs · ACB003-01_BCDM1057_MCT2_stim.fcs · ACB005-01_BCDM1062_MCT2_stim.fcs · ACB007-01_BCDM1067_MCT2_stim.fcs · ACB011-01_BCDM1093_MCT2_stim.fcs · ACB013-01_BCDM11104_MCT2_stim.fcs · ACB014-01_BCDM1110_MCT2_stim.fcs · ACB015-01_BCDM1112_MCT2_stim.fcs · ACB017-01_BCDM1116_MCT2_stim.fcs · ACB018-01_BCDM1119_MCT2_stim.fcs · ACB019-01_BCDM1123_MCT2_stim.fcs · ACB021-01_BCDM1139_MCT2_stim.fcs · ACB024-01_BCDM1194_MCT2_stim.fcs · ACB038-01_BCDM1535_MCT2_stim.fcs · ACB039-01_BCDM1585_MCT2_stim.fcs · ACB040-01_BCDM1595_MCT2_stim.fcs · BBC017_20211109_MCT2_stim.fcs · BBC017_20211117_MCT2_stim.fcs · BBC017_20211207_MCT2_stim.fcs · BBC017_20211215_MCT2_stim.fcs · BBC017_20211217_MCT2_stim.fcs · BBC017_20211222_MCT2_stim_2.fcs · BBC017_20211222_MCT2_stim_2.fcs-(1) · BBC017_20220302_MCT2_stim.fcs · BBC017_20220304_MCT2_stim.fcs · BBC017_20220318_MCT2_stim.fcs · BBC017_MCT2_stim_20211104.fcs · HCM109_BCDM1433_MCT2_stim.fcs · HCM114_BCDM1432_MCT2_stim.fcs · HCM116_BCDM1447_MCT2_stim.fcs · HCM117_BCDM1451_MCT2_stim.fcs · HCM118_BCDM1452_MCT2_stim.fcs · HCM119_BCDM1476_MCT2_stim.fcs · HCM120_BCDM1481_MCT2_stim.fcs · HCM121_BCDM1487_MCT2_stim.fcs · HCM122_BCDM1492_MCT2_stim.fcs · HCM123_BCDM1537_MCT2_stim.fcs · HCM125_BCDM1680_MCT2_stim.fcs · HCM126_BCDM1754_MCT2_stim.fcs · HCM127_BCDM1755_MCT2_stim.fcs · HCM128_BCDM1756_MCT2_stim.fcs · HCM129_BCDM1804_MCT2_stim.fcs · HCM130_BCDM1873_MCT2_stim.fcs |
