Experiment Overview
| Repository ID: | FR-FCM-ZYNE | Experiment name: | Apoptosis Flow Cytometry in human eosinophils after glucocorticoid exposure | MIFlowCyt score: | 98.67% |
| Primary researcher: | Fanny Legrand | PI/manager: | Luis Franco | Uploaded by: | Fanny Legrand |
| Experiment dates: | 2017-12-18 - 2018-04-20 | Dataset uploaded: | Jul 2018 | Last updated: | Sep 2018 |
| Keywords: | [APOPTOSIS] [Eosinophils] [glucocorticoids] | Manuscripts: | [29885264] |
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| Organizations: |
National Institute of Allergy and Infectious Diseases, Laboratory of Parasitic Diseases, Bethesda, MD (USA)
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| Purpose: | Verify transcriptional changes from in vivo glucocorticoid treatment. Measure cell death of human eosinophils isolated from blood before and 120 minutes after glucocorticoid administration. | ||||
| Conclusion: | There is no effect of glucocorticoids on the Annexin-V staining observed in human eosinophils | ||||
| Comments: | None | ||||
| Funding: | This research was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases (NIAID) at the National Institutes of Health. | ||||
| Quality control: | 2 Normal Donor were treated with 250 mg IV methylprednisolone. 1 hypereosinophilic syndrome (HES) patient was treated with 1 mg/kg oral prednisone. Eosinophils were isolated prior to and 120 minutes after treatment. The normal donor samples had an additional 60 minute time point. Autofluorescence and single-color controls of each fluorochrome were run for compensation | ||||
Experiment variables
| Doses | |
|---|---|
| · None | Patient_9_Baseline_None.fcs · Patient_9_Baseline_None_Unstained.fcs · Patient_11_Baseline_None.fcs · Patient_11_Baseline_None_Unstained.fcs · Patient_10_Baseline_None.fcs · Patient_10_Baseline_None_Unstained.fcs |
| · 1 mg/kg Prednisone | Patient_9_120min_Prednisone.fcs |
| · 250 mg IV Methylprednisolone | Patient_11_120min_Prednisone.fcs · Patient_11_120min_Prednisone_Unstained.fcs · Patient_11_60min_Prednisone.fcs · Patient_11_60min_Prednisone_Unstained.fcs · Patient_10_120min_Prednisone.fcs · Patient_10_120min_Prednisone_Unstained.fcs · Patient_10_60min_Prednisone.fcs · Patient_10_60min_Prednisone_Unstained.fcs |
| Conditions | |
|---|---|
| · Unstained | Patient_9_Baseline_None_Unstained.fcs · Patient_11_120min_Prednisone_Unstained.fcs · Patient_11_60min_Prednisone_Unstained.fcs · Patient_11_Baseline_None_Unstained.fcs · Patient_10_120min_Prednisone_Unstained.fcs · Patient_10_60min_Prednisone_Unstained.fcs · Patient_10_Baseline_None_Unstained.fcs |
| · Annexin V FITC/7AAD Stained | Patient_9_120min_Prednisone.fcs · Patient_9_Baseline_None.fcs · Patient_11_120min_Prednisone.fcs · Patient_11_60min_Prednisone.fcs · Patient_11_Baseline_None.fcs · Patient_10_120min_Prednisone.fcs · Patient_10_60min_Prednisone.fcs · Patient_10_Baseline_None.fcs |
| Individuals | |
|---|---|
| · Patient_9 | Patient_9_120min_Prednisone.fcs · Patient_9_Baseline_None.fcs · Patient_9_Baseline_None_Unstained.fcs |
| · Patient_10 | Patient_10_120min_Prednisone.fcs · Patient_10_120min_Prednisone_Unstained.fcs · Patient_10_60min_Prednisone.fcs · Patient_10_60min_Prednisone_Unstained.fcs · Patient_10_Baseline_None.fcs · Patient_10_Baseline_None_Unstained.fcs |
| · Patient_11 | Patient_11_120min_Prednisone.fcs · Patient_11_120min_Prednisone_Unstained.fcs · Patient_11_60min_Prednisone.fcs · Patient_11_60min_Prednisone_Unstained.fcs · Patient_11_Baseline_None.fcs · Patient_11_Baseline_None_Unstained.fcs |
| Timepoints | |
|---|---|
| · Baseline | Patient_9_Baseline_None.fcs · Patient_9_Baseline_None_Unstained.fcs · Patient_11_Baseline_None.fcs · Patient_11_Baseline_None_Unstained.fcs · Patient_10_Baseline_None.fcs · Patient_10_Baseline_None_Unstained.fcs |
| · 60 Minute | Patient_11_60min_Prednisone.fcs · Patient_11_60min_Prednisone_Unstained.fcs · Patient_10_60min_Prednisone.fcs · Patient_10_60min_Prednisone_Unstained.fcs |
| · 120 Minute | Patient_9_120min_Prednisone.fcs · Patient_11_120min_Prednisone.fcs · Patient_11_120min_Prednisone_Unstained.fcs · Patient_10_120min_Prednisone.fcs · Patient_10_120min_Prednisone_Unstained.fcs |
